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Chlorophyll, in various forms, is bound within the living cells of algae, phytoplankton, and other plant matter found in environmental water. Chlorophyll is a key biochemical component in the molecular apparatus that is responsible for photosynthesis, the critical process in which the energy from sunlight is used to produce life-sustaining oxygen. In general, the amount of chlorophyll in a collected water sample is used as a measure of the concentration of suspended phytoplankton, the magnitude of which can significantly affect the overall quality of the water. The use of the measurement of phytoplankton as an indicator of water quality is described in Section 10200 A. of Standard Methods for the Examination of Water and Wastewater (Standard Methods). The classical method of determining the quantity of chlorophyll at a particular site is to collect a fairly large water sample and analyze it in the laboratory. The procedure involves filtration of the sample to concentrate the chlorophyll containing organisms, mechanical rupturing of the collected cells, and extraction of the chlorophyll from the disrupted cells into the organic solvent, acetone. |
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